Construction of a plasmid interspecific transfer system in Bacillus species with the counter-selectable marker mazF
نویسندگان
چکیده
منابع مشابه
mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis
Here, we present a novel method for the directed genetic manipulation of the Bacillus subtilis chromosome free of any selection marker. Our new approach employed the Escherichia coli toxin gene mazF as a counter-selectable marker. The mazF gene was placed under the control of an isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible expression system and associated with a spectomycin-resistanc...
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This work describes the novel use of tolC as a selectable/counter-selectable marker for the facile modification of DNA in Escherichia coli. Expression of TolC (an outer membrane protein) confers relative resistance to toxic small molecules, while its absence renders the cell tolerant to colicin E1. These features, coupled with the lambdaredgam recombination system, allow for selection of tolC i...
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Counter-selectable markers can be used in two-hybrid systems to search libraries for a protein or compound that interferes with a macromolecular interaction or to identify macromolecules from a population that cannot mediate a particular interaction. In this report, we describe the adaptation of the yeast URA3/5-FOA counter-selection system for use in bacterial interaction trap experiments. Two...
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ژورنال
عنوان ژورنال: Journal of Industrial Microbiology and Biotechnology
سال: 2018
ISSN: 1476-5535,1367-5435
DOI: 10.1007/s10295-018-2038-0